Functional and Biochemical Characterization of DENV non-structural protein 1

EUVIRNA ESR-5

Project description
Dengue virus (DENV) is the most prevalent arthropod-borne virus, infecting up to 100 million individuals each year. DENV belongs to the Flaviviridae family and consists of a single positive strand RNA genome encoding for a polyprotein that is cleaved into 9 products. One of these, nonstructural protein 1 (NS1) exhibits a high degree of sequence homology amongst the flaviviruses, arguing for an important role in the viral replication cycle. In fact, NS1 plays a crucial role in controlling antiviral immune response, but in addition, this protein contributes to RNA replication by an as yet unknown mechanism.

The aim of this project is to characterize in detail the functional and biochemical aspects of DENV NS1. We will use reverse and forward genetic approaches to identify determinants that are crucial for the different functions of NS1. These studies will be complemented by proteomic analyses to identify viral and host interactants of NS1, thus providing important insights the composition of DENV replication complex.

Host institute
University Hospital of Heidelberg
Department of Molecular Virology
Supervisor: Prof. Ralf Bartenschlager

This position has been filled.