Biochemistry and function of nidovirus replicase proteins


Project description
Replicase polyprotein cleavage plays a crucial regulatory role in the replication of nidoviruses (arteri- and coronaviruses). The nidovirus main protease is responsible for up to 11 cleavages. Crystal structures have now been obtained for the main proteases of both arteriviruses (EAV, PRRSV) and coronaviruses (SARS-coronavirus, TGEV). In collaboration with P6, these proteases will be used for structure-based drug design and screening of potential inhibitors in in vitro and in vivo protease assays, and in infected cells. Furthermore, in collaboration with P5, reporter gene-expressing nidoviruses will be used to screen compound libraries aiming to identify specific inhibitors of virus replication and unravelling their mechanism of action.

Host institute
Leiden University Medical Center, Leiden, The Netherlands
Department of Medical Microbiology
Supervisor: Prof. dr. Eric Snijder

This position has been filled.